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Description
Human COL1A1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Sensitivity | 0.05 ng/mL | |||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Collagen Type I Alpha 1 (COL1A1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Collagen Type I Alpha 1 (COL1A1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Collagen Type I Alpha 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Type I collagen alpha 1 (COL1A1), also known as alpha-1 collagen, is a protein encoded by the COL1A1 gene. It is a fibrillar collagen found in most connective tissues, including cartilage. Collagen is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendons, skin, and the white part of the eye (sclera). The COL1A1 gene produces a component of type I collagen, called the proviso α1(I) chain. This chain combines with another proviso α1(I) chain and a proviso α2(I) chain (produced by the COL1A2 gene) to form the type I procollagen molecule. These three rope-like procollagen molecules must be processed by enzymes outside the cell. Once processed, they arrange themselves into long, thin fibers that cross-link with each other in the space surrounding the cell. The result of these cross-links is the formation of very strong, mature type I collagen fibers. The functions of collagen include rigidity and elasticity. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.1 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 5
No problems all the way to the end of the spool
Style: String Trimmer
I had an old Greenworks string trimmer that I found in the basement after I moved into my new home. Maybe it was just old, but the auto-feed didn't work well, the line was always running out, and I spent more time rewinding the spool than cutting down weeds. I had almost lost my faith in string trimmers entirely. You can spend $300 on one, but how much better are they? I didn't know. This Black & Decker was only $50, and although it's corded, my roommate convinced me it was worth not having to do the dance of recharging batteries, plus having full 110v power. Some (easy) assembly required out of the box, and this thing was basically plug & play. I did read through the owner's manual first, which gave amateur me some confidence through a few helpful tips. I use it not only for cutting down weeds, but also for cleaning out weeds from the cracks in my sidewalk, and the edger wheel is very helpful for that. More importantly, the line had no problems all the way to the end of the spool. Faith restored, there are good string trimmers in the world.
That being said, be aware that the line it comes with isn't very long. My lawn is medium-size, and it ran out about halfway through. The Black & Decker replacement spools are $10 / 30 ft (much longer), but it goes through line, so this could really add up. Replacing the spool was easy, and I was able to finish my lawn with plenty line to spare. A quick search on Amazon reveals off brand spools at $15 / 12-pack. I haven't tested them yet, but the price difference is so great that I'm going to give them a chance.
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Reviewed in the United States on August 19, 2023
★★★★★ 4
Handy until I can purchase a mower
Style: String Trimmer, Style: String Trimmer
I've used this thing heavily 3 times now. I need a mower but haven't had the spare money for one yet so this is what I'm using! My yard is just under 1 acre. I'ma list some pros and cons I've noticed in my good use..
Pros: it works.. really well! As you can see I live in Florida so grass grows super fast in our rainy season if I slack just 2 weeks oh my.. the power is strong, it's easy to work. Auto feed is a big help. I bought extra when I made this purchase I can go through about 1-2 rolls throughout the whole yard.
Cons: the wire. It gets in the way so much and my large yard I use a 200 ft cord but that's not always enough so I can't complete my job completely but enough I'm happy with. When the auto feed is low i noticed it constantly over feeds the line and that honestly hurts sometimes! It's and easy fix but after doing it 5 times you're over it. Id just put a new one on but I feel that's just a waste.
In the future I will be replacing mine to get rid of the wire.
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Reviewed in the United States on June 26, 2025
★★★★★ 5
Light Weight = Maneuverable
Style: String Trimmer
First time use today. It's works great. The light weight makes it so much more maneuverable than my very heavy 15 year old Echo weed line trimmer with heavy 58V battery. The Echo 58V battery died and did not want to spend $220 for new Echo battery with probably old tech battery. Debated on $300 EGO 56V trimmer and this corded 120VAC Black and Decker weed line trimer for $52. Also purchase a 50 ft AWG14 extension cord. The combination is terrific. Again the light weight is a revelation for me. The 6.5 Amp motor is plenty powerful. The thinner .065 line works well cut everything that needed wacking. The auto line feed works well. The length of tool fits me perfectly - I'm 5-4 so it may not be so good for tall folks. I extended to its max length. I wrapped the extension cord a few loops around the trigger handle and had no issues with cord staying on. Highly recommended with one could deal with extension cord. I don't see an issue with reliability and longevity. The electric motor seem well spec and strong. Really not much to go wrong with it. And no battery to deal with. For my very small yard it works great.
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Reviewed in the United States on March 5, 2026
★★★★★ 5
Great Light Weight String Trimmer and Edger!
Style: String Trimmer
I'm a 60 year old female and bought this because of the light weight. It was easy to put together, however, the instructions that came with it were terrible. It included only black and white images, with no written instruction. I found a great tutorial on YouTube that told how to assemble it and use it, and another on how to change the string. Buy extra string, so you'll have it ready! It does a great job on weeds and ivy. The edger is great. It is adjustable, but probably not the best for anyone taller than 5'6. It's noisy, but not so bad that it requires ear protection. I like that it's corded which makes it lighter and I don't have to fool with gas or heavy batteries.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Great weed wacker
Style: String Trimmer
Works great. Plenty of power. Did my weeding in a fraction of the time of my battery operated weed wacker
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Reviewed in the United States on May 15, 2026